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Currently, the method No. 12 – revision 2019 of the International Fruit and Vegetable Juice Association (IFU) is the accepted worldwide reference method for the detection of Alicyclobacillus spp. However, the verification of Alicyclobacillus in the lab is relatively time-consuming. In case of enrichment the results are available at the earliest after 10 days, but usually after almost two weeks.

An alternative method is the Soleris® ACB-109 (Neogen Corporation) which offers faster results which are just as reliable. This has been verified by a validation according ISO 16140-2:2016 (comparison of alternative against reference methods).

Via a two step enrichment of a 10 g sample, Alicyclobacillus can be detected already within two to four days using the Soleris ACB-system. For this purpose the sample is enriched for 2 days in BAT bouillon according to IFU No. 12 and afterwards transferred to the Soleris-Vial. To eliminate false positive results a molecular-biological rapid PCR test by Milenia Biotec GmbH will be used, directly out of the Soleris-Vial.

Detection of Alicyclobacillus within 96 hours
(Photo: Milenia Biotec GmbH)


Positive or negative – all important parameters for the evaluation of the sample are promptly provided:

  • Alicyclobacillus spp./10 g
  • Alicyclobacillus acidoterrestris/10 g
  • Potential guajacol formation

The Soleris ACB-system together with the PCR confirmation delivers the results quicker than the IFU No. 12 and is a reliable method for the qualitative verification of Alicyclobacillus spp. in fruit juices and concentrates.

In case of urgent Alicyclobacillus investigations the GfL Gesellschaft für Lebensmittel-Forschung mbH recommend the analysis by Soleris® ACB and Milenia® GenLine.